Antigen

Antigen Class

TFs and others

Antigen

DDX17

Cell type

Cell type Class

Pluripotent stem cell

Cell type

NTERA-2

Primary Tissue

Testis

Site of Extraction

lung

Tissue Diagnosis

Carcinoma, Embryonal

Sample

source_name

NTERA2

cell type

Pluripotent stem cells

chip antibody

DDX17 (Bethyl Laboratories, A300-509A)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Sheared chromatin was prepared by ChIP-IT Express kit (Active Motif, #53008) and immunoprecipitated by DDX5 or DDX17 antibody. The library preparation was performed using MicroPlex Library Preparation kit (Diagenode, C05010010).

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

195838466

Reads aligned (%)

86.5

Duplicates removed (%)

77.8

Number of peaks

9168 (qval < 1E-05)

hg19

Number of total reads

195838466

Reads aligned (%)

86.0

Duplicates removed (%)

78.7

Number of peaks

8966 (qval < 1E-05)